![]() They were able to detect the contamination of E. (1988) employed multiparameter light scattering to detect culture contamination, which is of great importance to the fermentation industry. However, stage of growth, growth medium, growth temperature, aeration, and the final dilution of the suspended medium can affect the reproducibility of this method. When an illuminated light from a polarized monochromatic light source shines on a sample (bacteria, for example), scattered light forms distinct patterns which could be used for identification and detection of bacteria. ![]() Light scattering technology differentiates samples based on refractive index, size, shape, and composition. Light scattering dates back many decades and has been used for many years in the semiconductor industry for the monitoring of defects on wafers. ![]() The latter technology is also known as BARDOT (bacterial rapid detection using optical scattering technology). There are two ELS-based systems under development for food pathogen detection: (i) direct identification of bacterial cells in liquid suspension and (ii) identification of bacterial colonies on solid media. Furthermore, the ELS method is nondestructive – i.e., it maintains the sample integrity during interrogation – and the signal measurement is instantaneous. Due to its unparalleled performance, ELS has been used in diverse science and engineering fields such as astronomy, semiconductor industry, and biology ( Bae & Bhunia, 2013). ![]() By analyzing the ELS signal, it is possible to provide a unique fingerprint of the organism under investigation without using specific labeling reagents such as nucleic acid or antibody probes, fluorophores, or enzymes. ELS signal strength is very high compared to other spectroscopic and inelastic scattering techniques (10 3–10 6 times higher than Raman signal). Bhunia, in High Throughput Screening for Food Safety Assessment, 2015 6.2.1 Physics of light scatteringĮLS is defined as an optical phenomenon that utilizes the characteristics of the spatial distribution of the scattered light with the same wavelength of illuminating light source. ![]()
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